The Civic and Diocesan Art Gallery of Spello preserves works owned by the parish of Santa Maria Maggiore and the Municipality of Spello, as well as pieces recently acquired thanks to the concession of private individuals.

The itinerary is divided into eight rooms , where the relationship between the city of Spello and other Umbrian artistic centers emerges, especially Orvieto, Spoleto, Foligno and Perugia. The works are placed between the 13th and 18th centuries, including a diptych by Cola Petruccioli, a Madonna with Child attributed to Andrea d'Assisi and the Triptych by the Maestro dell'Assunta di Amelia . The Triptych was dismembered at an unspecified time and the individual elements were reused as independent paintings for the altars of the collegiate church. Today he finds his original composition in the Pinacoteca. The central compartment featured a Madonna enthroned with Child , painted full-length like the lateral saints. In the early sixteenth century it was repainted: the artist in charge, whose identity is not known with certainty (some claim it is Pintoricchio, while today we tend to attribute it to Andrea d'Assisi known as the Ingenuity), cut the table, thus repainting a Madonna and Child in three quarters, but he left the background, the halos and the throne unaltered.

Some magnificent examples of Gothic and Baroque jewelery and an interesting section of medieval and Renaissance wooden sculpture are also exhibited in the Pinacoteca.