The path of the Museum of the Opera of the Cathedral of Prato winds through the Cathedral of Santo Stefano and the suggestive spaces of the Episcopal Palace around the Romanesque cloister. The museum houses works from the various churches of the Diocese of Prato, hosting some Renaissance masterpieces, such as the original marbles of the parapet of the pulpit by Donatello and Michelozzo and works by Filippo Lippi, Botticelli, Paolo Uccello, and Andrea della Robbia.

It also exhibits sculptures, paintings, illuminated choir books, and sacred goldsmithing from the 12th to the 20th century, as well as a small archaeological section from the Etruscan to the Renaissance period. The visit path also includes access to the transept of the Cathedral of Santo Stefano with the frescoed chapels. Among the five chapels, the most noteworthy are: The Main Chapel, the most famous, preserves one of the most important fresco cycles of the Renaissance, with the stories of Saint Stephen and Saint John the Baptist (1452-65) by Filippo Lippi; next to it, the Chapel of the Assumption frescoed in 1433-34 by Paolo Uccello, with stories of the Virgin and Saint Stephen; adjacent is the Vinaccesi Chapel which preserves frescoes by the Prato artist Alessandro Franchi (1873-1876).